resource source identifier pvsv g clontech (TaKaRa)
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resource source identifier pvsv g clontech
Resource Source Identifier Pvsv G Clontech, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 372 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pvsv+g/Retro-X+Universal+Packaging+System/pm41824454-675-2-6
Average 96 stars, based on 372 article reviews
Resource Source Identifier Pvsv G Clontech, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 372 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pvsv+g/Retro-X+Universal+Packaging+System/pm41824454-675-2-6
Average 96 stars, based on 372 article reviews
resource source identifier pvsv g clontech - by Bioz Stars,
2026-09
96/100 stars
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other:Article Title: TXNIP mediates LAT1/SLC7A5 endocytosis to limit amino acid uptake in cells entering quiescence Article Snippet: Plasmid Preparation:Article Title: Engineering stable and non-immunogenic immunoenzymes for cancer therapy via in situ generated prodrugs. Article Snippet: Engineering human enzymes for therapeutic applications is attractive but introducing new amino acids may adversely affect enzyme stability and immunogenicity.. Here we used a mammalian membrane-tethered screening system (ECSTASY) to evolve human lysosomal beta-glucuronidase (hBG) to hydrolyze a glucuronide metabolite (SN-38G) of the anticancer drug irinotecan (CPT-11).. Three human beta-glucuronidase variants (hBG3, hBG10 and hBG19) with 3, 10 and 19 amino acid substitutions were identified that display up to 40-fold enhanced enzymatic activity, higher stability than E. coli beta-glucuronidase in human serum, and similar pharmacokinetics in mice as wild-type hBG. Article Title: Targeting endogenous fatty acid synthesis stimulates the migration of ovarian cancer cells to adipocytes and promotes the transport of fatty acids from adipocytes to cancer cells Article Snippet: .. Briefly, 2×10 6 293T cells (Takara Bio USA, Inc.) maintained in DMEM plus 10% FCS were transfected in T25 cell culture flasks (Thermo Fisher Scientific, Inc.) by calcium phosphate co-precipitation with 5 μ g of the Moloney murine leukemia virus (MMLV)-derived retroviral expression plasmid pQCXIP (Takara Bio USA, Inc.) containing sequences for puromycin resistance and mCherry, and with 3.75 μ g p-gag-pol-gpt ( ) and 1.25 μ g Transfection:Article Title: Engineering stable and non-immunogenic immunoenzymes for cancer therapy via in situ generated prodrugs. Article Snippet: Engineering human enzymes for therapeutic applications is attractive but introducing new amino acids may adversely affect enzyme stability and immunogenicity.. Here we used a mammalian membrane-tethered screening system (ECSTASY) to evolve human lysosomal beta-glucuronidase (hBG) to hydrolyze a glucuronide metabolite (SN-38G) of the anticancer drug irinotecan (CPT-11).. Three human beta-glucuronidase variants (hBG3, hBG10 and hBG19) with 3, 10 and 19 amino acid substitutions were identified that display up to 40-fold enhanced enzymatic activity, higher stability than E. coli beta-glucuronidase in human serum, and similar pharmacokinetics in mice as wild-type hBG. Article Title: Targeting endogenous fatty acid synthesis stimulates the migration of ovarian cancer cells to adipocytes and promotes the transport of fatty acids from adipocytes to cancer cells Article Snippet: .. Briefly, 2×10 6 293T cells (Takara Bio USA, Inc.) maintained in DMEM plus 10% FCS were transfected in T25 cell culture flasks (Thermo Fisher Scientific, Inc.) by calcium phosphate co-precipitation with 5 μ g of the Moloney murine leukemia virus (MMLV)-derived retroviral expression plasmid pQCXIP (Takara Bio USA, Inc.) containing sequences for puromycin resistance and mCherry, and with 3.75 μ g p-gag-pol-gpt ( ) and 1.25 μ g Article Title: Long Terminal Repeats of Gammaretroviruses Retain Stable Expression After Integration Retargeting or Knock-In into the Restrictive Chromatin of Lamina-Associated Domains Article Snippet: .. The next day, cells were co-transfected by the viral genome, Gag-Pol ( ; ; ), and Article Title: Pauperization of Emerin from nuclear envelope during chromatin bridge resolution drives prostate cancer cell migration and invasiveness Article Snippet: EMD1 and EMD2 gRNA sequences were inserted via BsmBI restriction enzyme into plentiCRISPRv2_puro vector (Addgene, 52961) and applied for lentivirus preparation. .. Lentiviral particles were generated by co-transfecting 5 μg of the specific plentiCRISPRv2, 3.75 μg psPax2, and 1.25 μg Recombinant:Article Title: Engineering stable and non-immunogenic immunoenzymes for cancer therapy via in situ generated prodrugs. Article Snippet: Engineering human enzymes for therapeutic applications is attractive but introducing new amino acids may adversely affect enzyme stability and immunogenicity.. Here we used a mammalian membrane-tethered screening system (ECSTASY) to evolve human lysosomal beta-glucuronidase (hBG) to hydrolyze a glucuronide metabolite (SN-38G) of the anticancer drug irinotecan (CPT-11).. Three human beta-glucuronidase variants (hBG3, hBG10 and hBG19) with 3, 10 and 19 amino acid substitutions were identified that display up to 40-fold enhanced enzymatic activity, higher stability than E. coli beta-glucuronidase in human serum, and similar pharmacokinetics in mice as wild-type hBG. Retroviral:Article Title: Engineering stable and non-immunogenic immunoenzymes for cancer therapy via in situ generated prodrugs. Article Snippet: Engineering human enzymes for therapeutic applications is attractive but introducing new amino acids may adversely affect enzyme stability and immunogenicity.. Here we used a mammalian membrane-tethered screening system (ECSTASY) to evolve human lysosomal beta-glucuronidase (hBG) to hydrolyze a glucuronide metabolite (SN-38G) of the anticancer drug irinotecan (CPT-11).. Three human beta-glucuronidase variants (hBG3, hBG10 and hBG19) with 3, 10 and 19 amino acid substitutions were identified that display up to 40-fold enhanced enzymatic activity, higher stability than E. coli beta-glucuronidase in human serum, and similar pharmacokinetics in mice as wild-type hBG. Article Title: Targeting endogenous fatty acid synthesis stimulates the migration of ovarian cancer cells to adipocytes and promotes the transport of fatty acids from adipocytes to cancer cells Article Snippet: .. Briefly, 2×10 6 293T cells (Takara Bio USA, Inc.) maintained in DMEM plus 10% FCS were transfected in T25 cell culture flasks (Thermo Fisher Scientific, Inc.) by calcium phosphate co-precipitation with 5 μ g of the Moloney murine leukemia virus (MMLV)-derived retroviral expression plasmid pQCXIP (Takara Bio USA, Inc.) containing sequences for puromycin resistance and mCherry, and with 3.75 μ g p-gag-pol-gpt ( ) and 1.25 μ g Cell Culture:Article Title: Targeting endogenous fatty acid synthesis stimulates the migration of ovarian cancer cells to adipocytes and promotes the transport of fatty acids from adipocytes to cancer cells Article Snippet: .. Briefly, 2×10 6 293T cells (Takara Bio USA, Inc.) maintained in DMEM plus 10% FCS were transfected in T25 cell culture flasks (Thermo Fisher Scientific, Inc.) by calcium phosphate co-precipitation with 5 μ g of the Moloney murine leukemia virus (MMLV)-derived retroviral expression plasmid pQCXIP (Takara Bio USA, Inc.) containing sequences for puromycin resistance and mCherry, and with 3.75 μ g p-gag-pol-gpt ( ) and 1.25 μ g Virus:Article Title: Targeting endogenous fatty acid synthesis stimulates the migration of ovarian cancer cells to adipocytes and promotes the transport of fatty acids from adipocytes to cancer cells Article Snippet: .. Briefly, 2×10 6 293T cells (Takara Bio USA, Inc.) maintained in DMEM plus 10% FCS were transfected in T25 cell culture flasks (Thermo Fisher Scientific, Inc.) by calcium phosphate co-precipitation with 5 μ g of the Moloney murine leukemia virus (MMLV)-derived retroviral expression plasmid pQCXIP (Takara Bio USA, Inc.) containing sequences for puromycin resistance and mCherry, and with 3.75 μ g p-gag-pol-gpt ( ) and 1.25 μ g Expressing:Article Title: Targeting endogenous fatty acid synthesis stimulates the migration of ovarian cancer cells to adipocytes and promotes the transport of fatty acids from adipocytes to cancer cells Article Snippet: .. Briefly, 2×10 6 293T cells (Takara Bio USA, Inc.) maintained in DMEM plus 10% FCS were transfected in T25 cell culture flasks (Thermo Fisher Scientific, Inc.) by calcium phosphate co-precipitation with 5 μ g of the Moloney murine leukemia virus (MMLV)-derived retroviral expression plasmid pQCXIP (Takara Bio USA, Inc.) containing sequences for puromycin resistance and mCherry, and with 3.75 μ g p-gag-pol-gpt ( ) and 1.25 μ g Article Title: A model of human neural networks reveals NPTX2 pathology in ALS and FTLD. Article Snippet: .. To generate mTRE-GFP-FUS, GFP-FUS sequence from Hock et al. 27 was substituted into mTRE-TDP-43–HA via HiFi Kit. mTRE-TDP-43–HA and all other lentiviruses were then packaged into lentivirus via Lipofectamine 2000 (Invitrogen 11668019; 8.3 μl per ml final volume) co-transfection with CMV-Gag-Pol (Harvard dR8.91) and Article Title: A model of human neural networks reveals NPTX2 pathology in ALS and FTLD Article Snippet: .. To generate mTRE-GFP-FUS, GFP-FUS sequence from Hock et al. was substituted into mTRE-TDP-43–HA via HiFi Kit. mTRE-TDP-43–HA and all other lentiviruses were then packaged into lentivirus via Lipofectamine 2000 (Invitrogen 11668019; 8.3 μl per ml final volume) co-transfection with CMV-Gag-Pol (Harvard dR8.91) and Sequencing:Article Title: A model of human neural networks reveals NPTX2 pathology in ALS and FTLD. Article Snippet: .. To generate mTRE-GFP-FUS, GFP-FUS sequence from Hock et al. 27 was substituted into mTRE-TDP-43–HA via HiFi Kit. mTRE-TDP-43–HA and all other lentiviruses were then packaged into lentivirus via Lipofectamine 2000 (Invitrogen 11668019; 8.3 μl per ml final volume) co-transfection with CMV-Gag-Pol (Harvard dR8.91) and Article Title: A model of human neural networks reveals NPTX2 pathology in ALS and FTLD Article Snippet: .. To generate mTRE-GFP-FUS, GFP-FUS sequence from Hock et al. was substituted into mTRE-TDP-43–HA via HiFi Kit. mTRE-TDP-43–HA and all other lentiviruses were then packaged into lentivirus via Lipofectamine 2000 (Invitrogen 11668019; 8.3 μl per ml final volume) co-transfection with CMV-Gag-Pol (Harvard dR8.91) and Construct:Article Title: Long Terminal Repeats of Gammaretroviruses Retain Stable Expression After Integration Retargeting or Knock-In into the Restrictive Chromatin of Lamina-Associated Domains Article Snippet: .. The next day, cells were co-transfected by the viral genome, Gag-Pol ( ; ; ), and Generated:Article Title: Pauperization of Emerin from nuclear envelope during chromatin bridge resolution drives prostate cancer cell migration and invasiveness Article Snippet: EMD1 and EMD2 gRNA sequences were inserted via BsmBI restriction enzyme into plentiCRISPRv2_puro vector (Addgene, 52961) and applied for lentivirus preparation. .. Lentiviral particles were generated by co-transfecting 5 μg of the specific plentiCRISPRv2, 3.75 μg psPax2, and 1.25 μg |